Characterization of Aeromonas hydrophila through RAPD-PCR and SDS-PAGE Analysis

Aeromonas hydrophila isolated from fish (Labeo rohita), pond water, river water, raw meat of chicken and mutton and raw cow milk were characterized through Random Amplified Polymorphic DNA (RAPD) analysis and Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS PAGE) of cellular proteins. RAPD-PCR amplification of bacterial DNA was done by using ten random primers (OPA-01 to 10) and found some distinct banding pattern on agarose gel. RAPD profile was studied with each isolate and absolute polymorphism indicating its application as an ideal tool for molecular characterization. Other methods like morphological, serological and biochemical characterization gives contradictory results and total cellular protein profiling does not show any significant polymorphism for identification and discrimination.